CELL JOURNAL(Yakhteh), Vol 15, No 4, Winter 2014 282 Original Article In Vitro Maturation of Oocytes and Follicles and Increases Granulosa Cell Death 1 1 1 2 * Corresponding Address: P.O.Box: 6714967346, Department of Biology, Faculty of Basic Sciences,...
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CELL JOURNAL(Yakhteh), Vol 15, No 4, Winter 2014 282 Original Article In Vitro Maturation of Oocytes and Follicles and Increases Granulosa Cell Death 1 1 1 2 * Corresponding Address: P.O.Box: 6714967346, Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran Email: azadbakhtm_tmu@yahoo.com Abstract This study examines the effects of hydrostatic pressure on in vitro maturation (IVM) of oocytes derived from in vitro grown follicles. In this experimental study, preantral follicles were isolated from 12-day-old female NMRI mice. Each follicle was cultured individually in Alpha Minimal IVM and divided into two groups, control and experiment. In the experiment group follicles were subjected to 20 mmHg pressure for 30 minutes and cultured for 24-48 hours. We assessed for viability and IVM of the oocytes. The percentage of apoptosis in cumulus cells was determined by the TUNEL assay. A comparison between groups was made using the student’s t test. The percentage of
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