Responses of Atlantic cod Gadus morhua head kidney
leukocytes to phytase produced by gastrointestinal-derived
bacteria
Carlo C.
Lazado • Christopher Marlowe A.
Caipang •
Sanchala Gallage • Monica F.
Brinchmann •
Viswanath Kiron
Received: 1 July 2009 /...
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Responses of Atlantic cod Gadus morhua head kidney
leukocytes to phytase produced by gastrointestinal-derived
bacteria
Carlo C.
Lazado • Christopher Marlowe A.
Caipang •
Sanchala Gallage • Monica F.
Brinchmann •
Viswanath Kiron
Received: 1 July 2009 / Accepted: 8 October 2009 / Published online: 21 October 2009
Ó Springer Science+Business Media B.
V.
2009
Abstract This study identified phytase-producing
bacteria that were previously isolated from the
gastrointestinal tract of Atlantic cod, Gadus morhua
and determined its effect on head kidney leukocytes.
Out of the 216 bacterial strains tested, the two
phytase producers were identified as Pseudomonas
sp.
and Psychrobacter sp.
based on their 16S rDNA
sequence.
Crude phytase from these two bacterial
strains was produced employing the shake flask
method.
Even though the total protein of the crude
phytase was not significantly different for the two
bacteria, the phytase activity of the crude enzyme
produced by Pseudomonas sp.
(97.
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