Research Article
HPLC-DAD methodology for the
quantification of organic acids, furans and
polyphenols by direct injection of wine
samples
This article proposes a simple and sensitive HPLC method with photo-diode array
detection for the analysis of organic...
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Research Article
HPLC-DAD methodology for the
quantification of organic acids, furans and
polyphenols by direct injection of wine
samples
This article proposes a simple and sensitive HPLC method with photo-diode array
detection for the analysis of organic acids, monomeric polyphenols and furanic
compounds in wine samples by direct injection.
The chromatographic separation of 8
organic acids, 2 furans and 22 phenolic compounds was carried out with a buffered
solution (pH 2.
70) and acetonitrile as mobile phases and a difunctionally bonded C18
stationary phase, Atlantis dC18 (250 Â 4.
6 mm, 5 mm) column.
The elution was
performed in 12 min for the organic acids and in 60 min for the phenolic compounds,
including phenolic acids, stilbenes and flavonoids.
Target compounds were detected at
210 nm (organic acids, flavan-3-ols and benzoic acids), 254 nm (ellagic acid), 280 nm
(furans and cinnamic acid), 315 nm (hydroxycinnamic acids and trans-resveratrol) and
360 nm (flavonoids).
The RSD for
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