Extraction of oil from Jatropha curcas L.
seed kernels by
enzyme assisted three phase partitioning
Shweta Shah, Aparna Sharma, M.
N.
Gupta*
Department of Chemistry, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India
Received 15...
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Extraction of oil from Jatropha curcas L.
seed kernels by
enzyme assisted three phase partitioning
Shweta Shah, Aparna Sharma, M.
N.
Gupta*
Department of Chemistry, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India
Received 15 April 2003; accepted 16 October 2003
Abstract
Three phase partitioning has been evaluated for extraction of oil from Jatropha curcas L.
seeds.
This process consisted of
simultaneous addition of t-butanol (1:1, v/v) and 30% (w/v) ammonium sulphate to the slurry prepared from Jatropha seed
kernels.
Combination of sonication and enzyme treatment with a commercial preparation of fungal proteases at pH 9, led to 97%
oil yield within 2 h.
Keywords: Enzyme assisted three phase partitioning; Jatropha seed kernel oil; Three phase partitioning
1.
Introduction
Recently, we have described a new approach for
extraction of oil from plant materials, in general, and
soybean in particular (Sharma et al.
, 2002a).
In this approach, called three phas
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