DNA BARCODING
A PCR-based method for diet analysis in freshwater
organisms using 18S rDNA barcoding on faeces
EMMANUEL CORSE,* CAROLINE COSTEDOAT,* RE´ MI CHAPPAZ,* NICOLAS PECH,*
JEAN-FRANC¸ OIS MARTIN† and ANDRE´ GILLES*
*Aix-Marseille Universite´,...
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DNA BARCODING
A PCR-based method for diet analysis in freshwater
organisms using 18S rDNA barcoding on faeces
EMMANUEL CORSE,* CAROLINE COSTEDOAT,* RE´ MI CHAPPAZ,* NICOLAS PECH,*
JEAN-FRANC¸ OIS MARTIN† and ANDRE´ GILLES*
*Aix-Marseille Universite´, CNRS, IRD, UMR 6116 – IMEP, Evolution Ge´nome Environnement, Centre Saint-Charles, Case 36, 3
Place Victor Hugo, 13331 Marseille Cedex 3, †Montpellier SupAgro, INRA, IRD, CIRAD, Centre de Biologie et de Gestion des
Populations, Campus International de Baillarguet, CS30016, 34988 Montferrier-sur-Lez, France
Abstract
The development of DNA barcoding from faeces represents a promising method for animal
diet analysis.
However, current studies mainly rely on prior knowledge of prey diversity for a
specific predator rather than on a range of its potential prey species.
Considering that the
feeding behaviour of teleosts may evolve with their environment, it could prove difficult to
establish an exhaustive listing of their prey.
I
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